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The genomic sequences flanking the integration site of the transgene element were identified via an inverse PCR protocol.
Direct sequence analysis of individual PIAPs was then performed in order to identify the integration site.
The conventional forms were amplified with primers complementary to chromosomal sequences adjacent to the integration site.
Alternatively, or in addition, the integration site of the transgenes could modulate expression.
Their study suggested that HIV-1 integrase has the potential to select the integration site.
Several mechanisms have been proposed that chromatin accessibility influence the integration site selection [8].
The integration site is located at about 40 kbp apart from oriP.
Chromosomal integration of the DNA polymerase into ExCyto cells was confirmed by performing ExCyto PCR analysis of the integration site (Figure 1): amplification reactions contained primers flanking the integration site, dNTPs, and buffer, but no exogenous polymerase.
Although the mechanism for this process is poorly understood, both the integration site and the variable copy number at the integration site can influence the expression level [9] [11].
Table 1 summarizes the sequence-specific enrichments of Orc2 and Mcm7 at oriP and the integration site.
To identify the integration site, a genomic library was constructed for tgε26+/+ mice and screened using the Sstr5 probe.
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