Sentence examples for the inserting site from inspiring English sources

The site 0.5-1 cm outside and above the 3rd posterior sacral foramina will serve as the inserting site.

The needle is inserted at the inserting site inward and downward at a 30 45 degree angle to a depth of 50 60 mm.

After determining the inserting site, based on the amplification strategy), the Nsp2-GFP gene was amplified by overlap PCR using PRRSV infectious clone plasmid FL12 and gfp gene-contained plasmid pcDNA-EF1-GFP as the templates.

To construct GFP-PRRSV infectious clone, the inserting site for gfp gene should be selected properly so as to make gfp gene express efficiently and the GFP-PRRSV infection and replication in the host cells should not be significantly affected upon gfp gene insertion.

The specific Spe I site upstream and Xho I site downstream gfp inserting position was also considered during the inserting site determination.

Meanwhile, since the inserted gene is fused with viral nonstructural protein gene, the open reading frame of the viral coding region downstream of the inserted gene should not be changed after the insertion; the viral gene-encoded protein sequence downstream of the inserted site should not be interrupted.

The previous study also identified that most s2 sequences contained a pre-insert motif (MGTELGANTP) in the s region (SRP type I) before the insert site.

After purification, the inserted genes were sequenced using vector primers downstream and upstream of the insert site.

We attempted the 5′RACE method first because the mRNA upstream of the inserted site was expected to be trapped by the splice acceptor localized at the beginning of the gene trap vector.

However, the ribozyme activity of a group I intron appears to be facilitated by RNA chaperones in vivo; therefore, the efficiency of self-splicing could be dependent on the structure around the insert site or the length of the sequence to be inserted.

Analysis of the insert sites in the P. berghei genome showed that insertion occurred exclusively in the expected TTAA insertion site.