Sentence examples for the inserted site from inspiring English sources

Meanwhile, since the inserted gene is fused with viral nonstructural protein gene, the open reading frame of the viral coding region downstream of the inserted gene should not be changed after the insertion; the viral gene-encoded protein sequence downstream of the inserted site should not be interrupted.

We attempted the 5′RACE method first because the mRNA upstream of the inserted site was expected to be trapped by the splice acceptor localized at the beginning of the gene trap vector.

When we measure the amount of mRNA of A61, we used primers and probes for real-time PCR at both upstream (5′ primers in Fig. 4) and downstream of the inserted site (3′ primers in Fig. 4) and found a reduction of mRNA by both primers and probes (Fig. 6 A).

We could not find splicing from the upstream exons (exons 1 and 2) to the splice acceptor of the gene trap vector (Fig. 6 B), which suggests that the reduced amount of mRNA in the upstream of the inserted site was not because of the skipping of the upstream probe located at exons 3 and 4. In these cell lines, two possibilities were considered for their phenotypes.

The designed YS nanostructure with the large amount of nanosheets on its shell increased the inserted sites of Na ions due to large surface area.

The second reaction with primers (0168-1/0168-2 of pD1-1/p pD1-2d-2) of inserted site verified the wild-type was replaced completely.

The previous study also identified that most s2 sequences contained a pre-insert motif (MGTELGANTP) in the s region (SRP type I) before the insert site.

After purification, the inserted genes were sequenced using vector primers downstream and upstream of the insert site.

The site 0.5-1 cm outside and above the 3rd posterior sacral foramina will serve as the inserting site.

The needle is inserted at the inserting site inward and downward at a 30 45 degree angle to a depth of 50 60 mm.

After determining the inserting site, based on the amplification strategy), the Nsp2-GFP gene was amplified by overlap PCR using PRRSV infectious clone plasmid FL12 and gfp gene-contained plasmid pcDNA-EF1-GFP as the templates.