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Additional primers were designed on two other conserved regions of the α-gliadin gene to sequence the insert: one internal forward primer (designed on pos. 292 309), Fi1: 5'-CAA CCA TAT CCA CAA CCG-3', and one internal reverse primer (designed on position 599 615), Ri1: 5'-CA(C/T) TGT GG A/C) TGG CTT GGCTT'.
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Nonetheless, this does not alter the fact that WSSV copies in IHHNV-infected samples were much lower than those in the inserted ones.
Open the scissors and insert one of the scissor blades into the middle of the cork.
Regarding the integration rate, the previous works insert one bit per coefficient.
So, the procedure must insert one vertex to each θ+1 vertices in descending order, from vertex vl−t−1 in P θ.
DNA was digested with I- PpoI and run on a pulsed-field gel which showed that 2 had the modified BAC2LinkGm with the expected 61-kb insert (one shown in Fig. 4A).
In ebolaviruses, stuttering occurs on a run of 7 "U s in the negative-sense template to insert one or more additional "A"s in the positive-sense mRNA for the glyco-protein gene.
"Khatar," murmurs an onlooker quietly as Ismail slips his legs beneath the massive beam to insert one of his wedges.
And/or after that you can only put them in at a certain rate, you know; I think the rate is, you insert one and then a minute later you can insert another try, and then a minute plus a minute to the third try.
More recently, Volik et al. [ 15] proposed a procedure based on paired-end sequences that can detect much smaller variants, depending upon the type of sequence insert one employs.
He shot workmen labouring to insert one in the ground: "I find that really poetic, like those Americans putting their flag up.
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