Ms. St. Thomas emphasized that Mr. Hobbins, a 40-year veteran of the Smithsonian, had destroyed the initial transcripts of such board meetings as a matter of course since the 1980s, once the full formal minutes were prepared.
In the first stage of the analysis, the data from the initial transcripts for each of the PROMO groups was coded line-by-line.
The N0 arithmetic means from each biological replicate were divided by the corresponding NF (tissue injection) to obtain the relative N0 values corresponding to the initial transcript level of each gene in a given tissue and condition.
Hence, we removed these unnecessary sequences, accounting for approximately 52%% of the initial transcript set, ultimately obtaining a total of 232,702 contig sequences (including isoforms) for P. ginseng, with an average length of 1,752 bp and a maximum length of 20,589 bp (Table 2).
Therefore, the observation of a massive reduction of the initial transcript stock in our study was in line with the current understanding of events during oocyte maturation.
This analysis shows that degradation can reduce the transcript concentration to less than one third of the initial transcript abundance.
However, it has quickly become "obvious" that the linear process was a high-fidelity process which guaranteed the conservation of the initial transcript abundances [ 11].
In the case of human BDNF and antiBDNF, the transcripts could act as cis-antisense RNAs and generate siRNAs targeting one of the initial transcripts, as do the natural cis-siRNAs described for genes involved in salt tolerance in Arabidopsis thaliana [39].
There may be differences in the copying, or transcription, of the gene into RNA; in the processing of the initial RNA transcript into mRNA; in the control of mRNA movement to the cytoplasm; in the translation of mRNA to protein; or in the stability of mRNA.
