Sentence examples for the initial steps were from inspiring English sources

The initial steps were denaturation at 95°C for 10 minutes followed by 45 cycles of 15 seconds at 94°C, 30 seconds of 2 cycles annealing at 60°C, 58°C, 56°C, 54°C, 52°C, and 35 cycles at 50°C or 48°C and 45 seconds elongation at 72°C with final extension of 10 minutes at 72°C.

Once trained, the initial steps were performed with the animals walking with their hind legs moistened with water on the dyed leaves.

The initial steps were to diagnose the functioning of the existing HRS through the NHRS framework, prepare a policy for health research, inform and engage stakeholders and initiate research priority setting.

The initial steps were mainly carried out by the muscle biology research community, while the final discussion and refinement was carried out during a meeting with invited ontology developers.

If the initial steps are expanded, it could yield greater savings as well.

One element missing from the initial steps is any penalty for the doctors who commit some of the errors.

The initial steps are the adsorption of H2O2 to the Nb center followed by coordination of ethylene to the hydrogen peroxide.

The initial steps are the same as above, and employs HDEHP for extraction chromatography, but coprecipitates the mendelevium with terbium fluoride instead of lanthanum fluoride.

Mechanistically, the initial steps are poorly understood.

Here, we outline the 10 major steps of the algorithm; the initial steps are illustrated in Figure 1.

Potential issues are in achieving the initial steps are identified, and problem solving strategies are applied to these challenges [ 33].