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In the statistical test we used the highly-conservative Bonferroni correction where the initial significance threshold (0.05) was divided by the number of transcription factor combinations tested.
CDH1 did not exceed the initial significance levels set to triage differentially methylated candidate genes, but this gene was nevertheless chosen for analysis given its role as a common target for epigenetic inactivation in metastasis [ 21] and its functional relationship to other genes in the EMT network.
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In each analysis, Bonferroni correction for the number of markers tested was applied to an initial significance threshold of P = 0.05, setting a final significance threshold.
The observed allele pattern (heterozygous, homozygous P1, homozygous P2) frequencies across the progeny strains were then compared to this model using the multinomial exact test implemented in R. Bonferroni correction for the number of markers tested was applied to an initial significance threshold of P = 0.05, setting a final significance threshold.
Pearson chi-square was utilised for initial significance testing and this was followed by or checking using a Yates correction and Fishers Exact tests as necessary.
An initial significance level of p<0.02 (absolute t score>4.5) was once again chosen, but an additional requirement of a difference between the two means greater than 1.5 was applied to the methylation data as described above.
Thus, using an initial significance level of P = 0.05, after Bonferroni correction (two measures, two paired t-tests each), the adjusted significance level applied was P = 0.01.
When using VO2 peak as a covariate when analyzing BPV and HRV data, any initial significance (p < 0.05) remained, and results were not affected.
(c) Cryptographic security: if the two highest resolutions are very small, brute force attack becomes possible to guess the initial threshold and significance information.
A critical literature review and field surveys are used as the initial identification of significance places having regard to the remaining surviving evidence that can be promoted for conservation.
Analyses were performed at the whole brain level and the initial threshold for significance was a cluster-level statistic of P<.05FWEcorrected at the whole brain threshold of P<.001uncorrected.
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