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During infections lacking gp59, the initial replication forks from two different T4 origins actively replicated both leading- and lagging-strands.
The replication of animal mtDNA has been characterized in great detail, and in the original model each strand of the duplex DNA replicates at a different time, with the initial replication primed by a short transcript synthesized by the mitochondrial RNA polymerase [ 30].
Further studies point out the prominent role of FDCs, which are not BM derived cells, in the initial replication of prion [5], [6], [7].
These cells do not derive from hematopoietic precursors, and it has been clearly observed that mice defective for FDCs present a delay in the development of the clinical diseases, showing that the initial replication of infectivity in FDCs is critical for neuroinvasion [5], [6], [7].
Both of these loci were overlooked in the initial replication, despite being among the top 3000 (∼1%) SNP hits in the original screen.
The initial replication of this LDV mutant in and transmission between wild boars was likely slow until better host-adapted mutants were selected.
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From this initial replication site, the virus further spreads to the central target organs, spleen and liver.
HAART prevents the rapid initial replication needed to achieve populations required for effective chronic infection and, in concert with the aggressive immune responses; the virus is totally eliminated from the general circulation.
In mice, VEEV also disseminates to the spleen after initial replication in the draining lymph node (35).
ECTV usually enters its host through abrasions in the skin and after initial replication it migrates to the draining lymph node.
The results suggest that ALS and WBI not only increase tumour CFE by suppressing immunity to tumour growth but also "condition" host tissue (tumour bed) in such a way as to facilitate the survival, "take" and initial replication of grafted tumour cells before the rats recover from the immunosuppressive effects of these treatments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com