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The slowest step of the pipeline is phrap, followed by the initial repeat finder.
We propose that successive intrastrand deletions generated the initial repeat structure, and gene conversions increased the size of each tandem repeat locus.
After heating the sample again to room temperature, the initial repeat distance, and thus, the membrane structure is restored, which indicates a reversible process of excess water freezing (dehydration/hydration).
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With chromosome included as a factor in the model, initial repeat number remains the dominant predictor of mutation rate, although the estimated slope is reduced by about 25%.
Controlling for the effect of initial repeat number and motif type, the chromosomes were indistinguishable except for chromosome 4 which exhibits an elevated mutation rate (Z = 2.876, p<0.005).
Much of this variation can be attributed to the strong effect of initial repeat number.
Of the 12 persons classified as uninfected who had a repeat confirmatory WB test result that was indeterminate, 12/12 (100%) had a repeat negative EIA at the local laboratory, 8/9 (89%) had a repeat negative EIA at CDC, 6/6 (100%) had a repeat negative IFA, and 7/7 (100%) had a negative NAAT result on either the initial or repeat confirmatory specimen or both (Table 1).
While the initial experiments repeat successfully, others do not clearly support the conclusions.
Fish obtained from Selcoth Fish Farm in Moffat, Scotland were used for the initial and repeat range finding tests, while fish from Brow Well Fisheries Ltd., Skipton, England were used in the definitive test.
Substitute ρR into Eq. (8), a new z is got, and use new z to replace the initial value, repeat this process until the difference of two results is less than a certain minimum allowance.
The intra-class correlation between the initial and repeat AAC-8 scores on VFA was 0.87 (95% C.I. 0.80 0.92).
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