Sentence examples for the initial purification from inspiring English sources

To that end, we have previously reported the expression of a novel protein vaccine for malaria using the ExpreS2 Drosophila melanogaster Schneider 2 stable cell line system, however, a very low overall process yield (typically <5% recovery of hexa-histidine-tagged protein) meant the initial purification strategy was not suitable for scale-up and clinical biomanufacture of such a vaccine.

Reverse-phase Chromatography method (RP + C18) was performed for the initial purification and concentration of the filtered supernatant using Diaion HP-20 resin.

Related enzymes have been detected in other plant species that do not accumulate cardenolides, following the initial purification of P5ßR activity from Digitalis purpurea L. by Gärtner et al. 1994.

Additionally, the initial purification for each tagged protein kinase found a single peptide for different members of the targeting/adapter β-subunit family in the two different complexes.

We found it was important to use an inexpensive anion exchange resin for the initial purification because residual reactive dye can chemically attach to the free amines of the column.

Since the amino acid sequence was unknown the initial purification procedure included ammonium sulfate precipitation of the soluble protein fraction, anion exchange chromatography on DEAE-cellulose and size exclusion chromatography on Superdex 75 (Figure 1).

The extraction and initial purification was made by liquid-gel partitioning.

After initial purification the proteins were mixed and passed through a size-exclusion chromatography column together.

After initial purification, the TAP tag can be broken enzymatically for further processing and eventual MS analysis.

After initial purification, the proteins were enzymatically labeled with Alexa Fluor 488 (AF488) using Sfp phosphopantetheinyl transferase as described previously.

MAβ is released from ZAβ3 only after an initial purification of the complex has been achieved, limiting the potential for adverse effects during peptide preparation.