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Of the cytokines examined, Ag-specific interleukin-4 (IL-4) T-helper enzyme-linked immunosorbent spot (ELISpot) assays of the CD4bs-occluded group increased earlier (P = 0.025) during the inductive phase.
T cells are the primary players during the inductive phase of inflammatory arthritis [21].
Furthermore, experimental animal studies have shown that DCs pulsed with collagen are able to induce autoimmune arthritis after transfer to joints [6], suggesting that DCs play an active role during the inductive phase of inflammation.
Given that the latter is considered part of the normal negative feedback restriction of the inductive phase of the type I IFN response (reviewed in [20]), we favored the conclusion that sustained expression of ISG transcription in a subset of BL lines, i.e., beyond several hours post induction, is an aberrant response.
The related boxplots in Fig. 4E and F of the slope values obtained from the recordings shown in Fig. 4A D depict the differences between the inductive phase of the early LTP and the late LTP effects of the cage controls versus sham and EMF exposed animals.
We use mathematical modelling to investigate the effect of imperfect adherence during the inductive phase.
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Finally, the use of an existing framework deductively, and the subsequent inductive phase to accommodate extraneous data, offers a transparent mechanism by which a team might document any added, and potentially innovative, contribution made by data derived for the review.
Taken together, these results suggest that vitamin D3 may regulate the differentiation and/or migration of CD4+ T cells in the EAE inductive phase.
However, the hTNFtg animal model has its limits, since it bypasses the autoimmune inductive phase, which is seen in other arthritis models such as collagen-induced arthritis or adjuvant arthritis, as well as in human disease.
These data suggest that enhanced IL-10 production following treatment with vitamin D3 may regulate TH17 differentiation via an autocrine effect in the EAE inductive phase.
EAE is a commonly studied animal model of acute central nervous system inflammatory demyelinating disease, and it is believed that autoreactive T cells and proinflammatory cytokines play key roles in the early inductive phase and later paralytic stages of EAE pathogenesis [16], [30], [31].
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