He (she) called one out of two addressees, then pointed at an object and then the designated addressee named the indicated target (Figures 1a and 1b).
The comparative CT method was used to quantify targets; CT values were normalized to β-actin levels in each tissue and expressed relative to the indicated target in the indicated tissues.
Yeast cell protein extracts containing the indicated target proteins were passed over the amylose resin, and proteins bound to MBP-Ulp1(3), MBP-Ulp1(3)(C580S) or MBP-Ulp1(3)(C580S)ΔSBS were eluted with 100 mM maltose or SDS-PAGE sample buffer.
To investigate whether hsa-miR-520h influenced cell proliferation, cell cycle, or apoptosis of PANC-1 cells by inhibiting translation of the indicated target ABCG2 mRNA, we transiently transfected PANC-1 cells with hsa-miR-520h mimics for 24 h.
In order to determine the transcriptional outcome of p53 binding to NGF-responsive target regions described above, RT-PCR was performed to compare mRNA levels for indicated targets among naïve and differentiated PC12 cells.
The killing assay was performed by co-culturing CD8+ lymphocytes with CFSE-labeled control EL4 cells (ATCC, Manassas, VA, USA) or the OVA-expressing EL4 derivative cell line E.G7-OVA (ATCC) at the indicated effect: target cells ratios.
The two response types compare an aimed pointing movement, made by the arm to an indicated target location, with a keypress response that is, a finger movement to press an indicated response key, with one key uniquely associated with each finger using a spatially compatible mapping.
For transient transfection, cells were transfected with indicated target vectors using X-tremeGENE reagent (Roche) according to the manufacturer's instructions.
The DNA binding of His-RcsB-P His-RcsB-P His-RcsB-PsA (involved in EMSA and DNase I footpresence) of that of His-RcsB-P alone (EMSA) to indicated target DNA probes was tested.
