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All strains used in this study were performed in the indicated backgrounds (Table S1).
The model allows for differing cut-off specifications using the indicated criteria (Table 2).
Assay A: Only the natural amino acid was included in this charging assay at the indicated concentration (Table 3).
E. coli ΔsodAB and Hpx− strains transformed with the indicated plasmids (Table S1) were used to determine intracellular ROS by flow cytometry.
(18S) at the indicated temperatures (Table 1) and elongation at 72°C for 1 min After the last cycle, cell samples were incubated for an additional 10 min at 72°C.
Gene modules (gene sets) were collected from Gene Ontology [ 27], MSigDB [ 28] and the supplementary datasets of the indicated publications (Table 2).
The cycling protocol was 30 s denaturation at 95°C, 30 s annealing at the indicated temperature (Table 2), 50 s elongation at 72°C.
The adults were removed, and the eggs were incubated at the indicated temperatures (Table 1) for 48 h before dauer formation was scored under a Leica S6 stereomicroscope (Leica, Wetzlar, Germany).
The qRT-PCR was performed with 45 cycles composed of 30 s denaturation at 95 °C, 30 s annealing at the indicated temperature (Table 1) and 30 s extension at 72 °C after 10 min of initial denaturation at 95 °C.
20 HCC cells, seeded the day before at 3 × 10 cells/well into a 96-well plate, were serum-starved for 4 hours and then treated with selected nutraceuticals or DMSO at the indicated concentration (Table 1) for 2 hours.
The qRT-PCR was performed with 45 cycles composed of 30 sec denaturation at 95°C, 30 sec annealing at the indicated temperature (Table 2A) and 30 sec extension at 72°C after 10 min of initial denaturation at 95°C.
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CEO of Professional Science Editing for Scientists @ prosciediting.com