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ELISA competition assays were performed by incubating either each of the 18 selected human affinity-purified antibodies, or antibodies elicited in mice, together with each of the 18 antigens over the indicated range of concentrations for 30 minutes at room temperature prior to addition to the ELISA peptide-coated plate wells.
For each section, the percentage of positive cells at a given distance was calculated by dividing the number of Fucci-expressing cells by the total number of nuclei included in the indicated range of distance.
The indicated range of water temperatures during the Dutch seasons, however, could help with translating our findings to other climates.
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We note that concentrations higher than 32 μM bosentan or 10 μM K-8794 induced nonspecific AP-TGF-α release responses, and thus we used the indicated ranges of the antagonists.
The midpoint of the indicated range for both years applied and days per year applied were used to calculate the exposure metrics.
After symmetrization, we observe remarkably small variations: in average, they do not exceed 10% of the interface in the indicated range for cut2.
However, one can directly compare model values and experimental estimates only at altitudes within the indicated range, where measurements of the appropriate parameters are carried out.
The results of research on ocean acidification, the sensitivity analysis and the aggressive assumptions about adaptation taken in this study suggest a CO2 stabilization target at the upper end of the indicated range would likely fail to avoid degradation of most coral reef ecosystems.
Chinese chip maker Advanced Semiconductor Manufacturing Corp has priced its IPO at the mid-point of the indicated range, allowing it to raise HK$650.7 million ($84 million) before the April 7 trading debut.
Bankers handling the Orange share sale were last night pushing for an offer price at the bottom of the indicated range, hoping a low price would stimulate demand in the weakened telecoms market.
DEI was priced at $16, in the middle of the indicated range, when the company filed to go public, but the underwriters did not do a good job of estimating investor interest.
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