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Throughout this paper is a generic constant, not necessarily the same at each occasion (it will change from line to line), which depends in an increasing way on the indicated quantities.
For the recombinant protein inhibition assays, slides were preincubated with the indicated quantities diluted in cytoadhesion medium for 1 h at 37°C.
The next day, fresh medium or medium containing the indicated quantities of drug was applied to the dishes.
Fluorescently labelled DNA substrate (3 nM) was incubated at 30 °C with the indicated quantities of Srs2 in buffer E (30 mM Tris pH 7.5, 1 mM DTT, 0.1 mg/ml BSA and 100 mM KCl) for 10 min.
Fluorescently labelled DNA substrate (6 nM) was incubated at 30 °C with the indicated quantities of Srs2ΔC, Rad5 or Mph1 in buffer D (25 mM Tris pH 7.5, 1 mM DTT, 0.1 mg/ml BSA, 50 mM KCl, 7.5 mM creatine phosphate, 11.25 μg/ml creatine kinase, 2.5 mM MgCl2 and 2.5 mM ATP) for 15 min.
Fluorescently labelled DNA substrate (3 nM) was incubated at 30 °C with the indicated quantities of Srs2 in buffer H (30 mM Tris pH 7.5, 1 mM DTT, 0.1 mg/ml BSA, 100 mM KCl, 20 mM creatine phosphate, 20 μg/ml creatine kinase, 2.4 mM MgCl2, and 2 mM ATP) for 10 min.
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Posters were not put up in the indicated quantity, the truck was not driven through the village playing the jingle and the intervention team did not gather men for group meetings in the evenings.
(D ) Time spent licking was determined over 10 min following hindpaw injections of the indicated capsaicin quantities in 10 μl vehicle for WT (n = 9, 9, 20, 20, 18, 20, 20, 20, 20, and 9 from low to high capsaicin) and Slo2 dKO (n = 10, 10, 11, 9, 14, 26, 13, 18, 10, and 10) genotypes.
Each PMFS was made by mixing a mixture sample at an indicated quantity with its exogenous reference ligand (BMPL or BPDEDA) at a fixed quantity, both in methanol, to have a final reference ligand at 200 μM in each PMFS.
According to Dr. Cooperman's written testimony, the most common problems are supplements that lack adequate quantities of the indicated ingredients and those contaminated with heavy metals.
This quantity is the indicated horsepower of the engine, so called from an instrument known as the engine indicator, which is used to measure the pressure on the piston and thus calculate the power developed in the cylinder.
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