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We used the Barnes–Hut implementation of t-SNE from the Rtsne-package on the indicated numbers of ROSE-selected probes with no prior PCA, and visualized the results as 2D or 3D plots.
Phoenix cells were transfected with the indicated plasmid vector(s) and a murine packaging "helper" construct, φ, using Fugene transfection reagent (Roche, West Sussex, UK) following the manufacturer's protocol.
For the generation of retroviruses, the Phoenix-Eco packaging cell line was transfected with the indicated pBabe-puro/zeo expression plasmids using the calcium phosphate method.
Cook al dente, following the timing instructions on the package but checking a minute before the indicated cooking time.
4. Cook the pasta al dente, following timing instructions on the package but checking a minute before the indicated time.
Cook al dente following the timing directions on the package but checking about a minute before the indicated time.
Cook al dente, following the timing instructions on the package but checking the pasta a minute before the indicated time.
Enrichment analysis was performed using the R/Bioconductor GOstats package for known transcripts displaying statistically significant changes in the indicated mutant vs wild-type strain (absolute log2 fold-change > 1.5, FDR-adjusted p -value < 0.05).
Cook al dente, until firm to the bite, following the cooking instructions on the package but checking the pasta a minute or two before the indicated time.
Data were presented as mean ± SD for the indicated number of independently performed experiments and the data were analyzed using the SPSS package for Windows (Version 11.5).
Please follow the indicated word limits.
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