Based on the indicated use of instruments, we concluded that this pressure-induced discomfort is a result from the use of scissors handles.
Nuclear magnetic resonance (NMR) spectra were obtained on Varian 400 or Bruker 500 MHz instruments in the indicated solvents.
Detection was performed using a LiCor Odyssey instrument, using the indicated primary antisera with corresponding LiCor secondary antibodies.
Raw fluorimetric data (e.g., as in figures) are the averaged instrument readings for the indicated number of replicate wells.
Oxygen consumption and CO2 production were measured in individual mice at the indicated age using an Oxymax apparatus (Columbus Instruments, Columbus, OH, USA).
After attachment, cell migration or invasion (coating of top well with 2 μg of Matrigel) toward NIH-3T3-conditioned media was continuously monitored in real time for the indicated times using the xCELLigence RTCA DP Instrument (Roche Applied Science).
This quantity is the indicated horsepower of the engine, so called from an instrument known as the engine indicator, which is used to measure the pressure on the piston and thus calculate the power developed in the cylinder.
RT-PCR was carried out using 1 μL of cDNA per sample, HotStart master mix (SuperArray, Frederick, MD) and 0.25 μM each primer (IDT) in a GeneAmp 9700 PCR instrument (Applied Biosystems, Foster City, CA) for the indicated number of cycles.
In the indicated experiments, RR was injected to a final concentration of 30 μM using a Nano injector (VWR instrument, Sugar Land, TX).
Cook al dente, following the timing instructions on the package but checking a minute before the indicated cooking time.
