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Cells were first infected with the indicated RNA virus and then infected with VACV immediately (T = 0) or at the indicated hours (T = 2 24) post-RNA virus infection.
(b and c) qRT-PCR analysis of the expression of miR-200c-3p miR-200c-3p miR-200c-3pcells andthe miR-141-3p miR-141-3p miR-141-3pwinh H1N1 virus, H5N1 virus (MOI=4) or A549ontrol.
A549 cells were pre-treated with 5 μM B[a]P for 9 days and then incubated in combination with 1 μM vorinostat for the indicated hours.
Flagellum length was measured after the indicated hours upon RNAi induction using the axoneme marker Mab25 (n = 200 for each induction time).
Synchronous L1 stage animals were added to normal food (OP50 bacteria) and incubated at 20°C then collected at the indicated hours with food.
The cell monolayer was scratched with micropipette tips and images were captured at the indicated hours after wounding using a Nikon microscope.
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After drug exposure for indicated hours, the MTT solution (5 g/L) was added to the plates.
Hep3B and SMMC-7721 cells were stimulated with TNF-α in the presence of 3-MA for the indicated times (hours).
Shown are posterior trunk fragments cut from wild-type animals and regenerated for the indicated times: hours or days post-amputation (hpa and dpa, respectively) and subjected to double FISH with zpuf-6 and AGAT-1.
(a c) HepG2 cells were treated with PA for indicated hours and analysed by immunoblotting (images with black bands) and RT PCR (images with white bands).
(a c) HepG2 cells stably transduced with Sestrin2 shRNA (sh-Sesn2) were treated with PA for indicated hours (a) or 6 h (b).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com