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Cells were stained with the indicated antibodies (Additional file 2: Table S1) according to the manufacturer's instructions and analyzed using the MACSQuant™ Analyzer (Miltenyi Biotec) (Additional file 3: Figure S1).
Systolic blood pressure (SBP) and diastolic pressure (DBP) were measured 0.5 h after the administration of Rg1 at the indicated time (Additional file 4: Figure S4) using the tail-cuff method.
The cellular morphology following VFL treatment was analysed by phase-contrast microscopy in the indicated cell lines (Additional file 1 and Figure 2).
E. coli BL21 (DE3) cells were transformed with the indicated plasmid pETC02 (Additional file 1: Figure S1), while OtrC expression was induced by IPTG and analyzed by Coomassie blue staining of the SDS-polyacrylamide gels (SDS-PAGE) and Western blotting.
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mus-sample-type-name (format) e.g. "16-bit big endian linear" create a new sound file with the indicated attributes, return "fd" mus-oss-set-buffers (num size) in Linux (OSS) sets the number and size of the OSS "fragments".
DEI was priced at $16, in the middle of the indicated range, when the company filed to go public, but the underwriters did not do a good job of estimating investor interest.
Each individual reaction contains 1.0 pmole of the indicated primers (provided in Additional File 4) and 1 μl of 5-fold diluted single-stranded cDNA.
All final primer sets showed homoeolog-specific amplification from plasmid DNA templates at the indicated optimal annealing temperatures (see Additional files 11 and 12).
For real-time qPCR, reactions were performed using SsoFast EvaGreen or SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), according to manufacturer's recommended conditions, with primers targeted to the indicated region of selected genes (Supplementary file 1) on a CFX96 Real-Time PCR detection system (Bio-Rad).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com