To liberate untagged target proteins into the supernatant fractions, 300 500 µL lysis buffer was added to the Ni2+-NTA beads containing CPD-His6 fusion proteins and the indicated amount of inositol hexakisphosphate (InsP6, Calbiochem) was added.
The calcium phosphate method was used to transfect 0.5 µg of HIV-CAT, 50 ng of SV40-LUC as an internal control for transfection efficiency, and the indicated amount of either pcTat or a CMV-driven empty vector.
no addition; addition of the indicated amount of additives.
PCR fragments and genomic DNAs were used as templates for ligation in the indicated amount.
The indicated amount of DNA in Tetrahymena was determined for vegetative cells.
The indicated amount of protein lysate was separated by SDS-PAGE and transferred to Immobolin-P.
HeLa cells were transfected with 500 ng of neomycin reporter plasmid and the indicated amount of helper plasmid expressing transposase.
For luciferase assays, the cells were cotransfected with a reporter plasmid and the indicated amount of expression vectors.
Experiments were done the indicated amount of times, and the data were analyzed using a Student's paired t-test.
After incubating, the samples were immediately placed on ice, and the indicated amount of TPCK-treated trypsin (Worthington) was added to each sample.
After 24 h of incubation the medium was removed, and the indicated amount of siRNA diluted in 100 μl DMEM with 10% FCS was transferred to each well.
