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Although one could argue that a band of the incorrect size is not a positive result per se, an assay by definition should produce a binary band or no band result.
Primer pairs that failed to produce a product, produced a product of the incorrect size or produced multiple products were discarded, and new primers were designed and re-ordered.
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The DNA inserts with an incorrect size were considered as recombined, and discarded.
In our experience, a predominance of unwanted products of incorrect size is observed if the same pair of primers is used in both the emulsion PCR stage and the secondary amplification.
Conducting inference under the incorrect bottleneck size can generate misleading results.
Interestingly, contrary to the incorrect EMG size and correction time, its parameters do not allow for prediction of the detection outcome, suggesting that it is likely a consequence, rather than a cause, of detection.
It is estimated that 80% of women wear the incorrect bra size, for example.
The bands in Figure 5 are dismissed due to incorrect size, but what are these products?
The sequences with an early stop codon or with an incorrect size were considered as recombined.
Reference-free 2D classification in IMAGIC was subsequently used on the data sets to remove averages with blurred appearance or incorrect size.
This stands in contrast to previously published investigations of Bcl-G mRNA and Bcl-G protein expression, although the latter used antibodies for western blotting that reveal a band of incorrect size for mBcl-G.
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