Despite the improved sampling (with N = 1,...,15), artifacts around gold particles from the missing pyramid (or cone) are still slightly visible in any FBP reconstruction.
The improved sampling of butane rotamers in states 0 and 1 by using sesTI is evident across λ and the replicas.
It may be that the improved sampling achieved by Rinke et al. (2013) has helped to stabilize the position of these previously problematic taxa (Brochier-Armanet et al. 2011) in phylogenetic trees.
This trend is now more apparent because of the improved sampling of genomes currently available; closely related Microsporidia such as the available Nematocida strains are highly syntenic (∼99.7%), but this value drops to below 20% among the sampled species that are more distantly related to each other.
With this improved sample, the atpI phylogeny was no longer in significant conflict with the organismal tree; instead, relationships among Triticum, Oryza, and Saccharum/Zea were entirely unresolved under an all nucleotide position ML model (Additional File 6).
The combination of improved sample preservation approaches and advancements in the field of electron tomography and high-resolution fluorescence microscopy are expected to enlighten kinetochore organization at increasing resolution.
The facilities and activities of FaME38 are described with particular emphasis on the use of improved sample positioning equipment and procedures.
Two samples were used: the initial one, composed of 117 case studies, without a pre-selection of the cases analysed, and an improved sample, composed of 195 claddings, with more complete and reliable data.
Nonetheless, we anticipate that continued improvements in instrumentation, combined with improved sample preparation, will provide additional sequencing depth and speed in the future and the present study illustrates how this can be used to produce a more comprehensive mapping of gene expression and regulation during fundamental biological processes.
