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Improved versions (v2) of the X. tropicalis genome-wide microarray design and the promoter microarray design are available at the authors' web site (http://www.ncmls.nl/gertjanveenstra).nl/gertjanveenstra
Appropriate microarray design is fundamental to the success of these experiments.
The implementation of our design is done in two steps.
Two different microarray designs were used.
A detailed description of the microarray experimental design is shown in Supplementary Table S1, sheet "MA design".
Hence, the quality of the microarrays design which includes selecting short Oligonucleotide sequences (probes) to be affixed on the surface of the microarray becomes a major issue.
DK designed the implementation of the study.
A challenge to the implementation of single-species microarrays in comparative transcriptomics is the identification of unbiased probes.
An assessment of ease of implementation of each design paper was made.
34 The case study design is totally appropriate for the analysis of complex intervention implementations.
The microarray was designed with four replicates of each probe distributed across the array.
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