In order to assess the impact of the Mu insertion into the EXPB1 gene on Zea m 1 production, maize pollen was collected in July 2004 from EXPB1/EXPB1 and expb1/expb1 plants grown at The Pennsylvania State University Agricultural Experiment Station at Rock Springs PA (near State College, PA), cleaned by passing through a series of sieves, and stored separately at −80°C.
To evaluate the impact of the genetrap insertion on Rab18 mRNA expression, reverse transcriptase PCR was performed on cDNA that had been extracted from embryonic day (E 11.5 embryos.
RT-qPCR was also performed to monitor the impact of the T-DNA insertion on FPGS Os03g02030 mRNA abundance using cDNA specific primers, 3gE1_for and 3gE1 1_rev (Fig. 4).
Third, we only investigated the impact of the central line insertion bundle on CLABSI but did not evaluate the effect of the central line maintenance bundle.
Despite the impact of these insertions on viral fitness, live imaging revealed that replication of tagged-HCV genomes induced specific redistribution of the mCherry-tagged-MS2-Coat protein to motile and static foci.
To investigate the impact of the R11 promoter trap insertion allele on the CBX5 transcript profile, exon connection experiments were developed, using RT PCR.
As very few annotated genes are located in these regions, the impact of the effective lack of insertions in these regions on functional mutagenesis is likely to be minimal.
The impact of the myb28-1, myb29-2, myb29-2, myb76-1 and myb76-2 insertions on the glucosinolate pool in seeds was measured on seeds derived from the plants on which glucosinolates in leaves had been measured.
In this study, we used likely proxies for respiratory infections in children, such as antibiotic use and ventilation tube insertions (VTI), to estimate the impact of the vaccine on a national level.
