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Metabolomics complements the immunological studies.
The behavioural results were analysed by use of 2-sample t-test (Minitab ver. 14, Minitab Inc .. Data from the immunological studies were analysed by use of one-way ANOVA and two-sample t-test.
Indeed, data analysis showed a statistical correlation between PCDAI in CD patients with P2 and P7 peptide cytokine production that proved to be relevant in the immunological studies, i.e., the HSP60/65-derived HSP60/65-derivedggered a peptidenthatoinflammatriggeredkine induction.
EP carried out the immunological studies.
AS participated in the immunological studies.
MN carried out the immunological studies.
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CJN contributed to the immunological study design, data analysis and contributed to the manuscript writing.
Seven RA patients starting anti-TNF agents were used as controls for the immunological study.
Serum samples were sent to each laboratory at convenient time intervals (Madrid for the immunological study, Granada for the biochemical, metabolic and vitamin studies and Pamplona for the genetic study).
Indeed the authors of the immunological study indicated that the epitope for the antibody has highest sequence similarity to A. millepora nuclear receptor 8, but phylogenetic analyses indicate that AmNR8 is most similar to TR2/4 [ 4].
The limitations of Study 3 are the restricted sample size (n = 200), which is the consequence of the high costs of the immunological study (approximately 600€/subject) and the labour intense data collection process involved in measuring physical activity using the accelerometer.
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