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The identified time-dependent expression patterns from pathogen and host cells were confirmed by MIQE-conform qRT-PCR [ 35] with three independent biological replicates.
Cross-sectional surveys only identified time-dependent loci, but not provided insights into how such factors influenced traits over time [ 27, 45].
From the present study, we conclude the importance of identifying time-dependent prognostic factors.
In other words, the first ANOVA evaluates along-stream differences in water quality for each period, and the second ANOVA identifies time-dependent changes at individual sampling sites.
Nonstationary GEV analysis identifies time-dependent changes in the distribution of spring extremes for snowmelt dominated and glacially dominated systems.
This prompted us to identify time-dependent inhibitors of the FabI enzymes from other organisms including that from the important human pathogen Mycobacterium tuberculosis (InhA) and to explore the mechanistic basis for slow-onset inhibition.
The hybrid/inverse identification method is proved to be promising in identifying time-dependent interfacial parameters of adhesive bonded structures.
We employed this technique to identify time-dependent transcription patterns of interacting HeLa-S3 and SL1344 cells subsequent to infection of the host cells.
We also used DNA microarrays to identify time-dependent changes in gene expression during RA-induced growth arrest and differentiation of B16 melanoma cells.
This new nonradioactive ECL-IAA assay can define earlier onset of prediabetic autoimmunity and thus may help identify time-dependent events triggering islet autoimmunity.
Our aim was to identify time-dependent and growth factor-specific changes in gene expression associated with long-term EGF and HRGβ1 (hereafter HRG) stimulation of a model HMLEC system.
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