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At this stage, we eliminated reporters for which no identifiable transcript identifier could be found in the annotation file, or for which the identified sequence could not be found in either database.
Protein modeling shows that the canonical palm subdomain architecture of poliovirus (ssRNA+) RdRp could accommodate the identified sequence permutation through changes in backbone connectivity of the major structural elements in three loop regions underlying the active site.
Based on the identified sequence preferences, a cleavage site recognized by both HIV-1 and M-MuLV enzymes was introduced into a sequence that was otherwise resistant to RNase H.
Assessments of the effect of the identified sequence variants have been limited in populations of other ethnicities.
Interestingly, this search identified RE1-silencing transcription factor (REST) as the closest transcription factor (P-value = 1.7e-5) that could potentially bind the identified sequence (Fig. 4D) [30].
It is possible therefore that the identified sequence is not only key to female gonadal development but is regulated in both female and male sex determination.
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Dashed lines connect the identified sequences to the position in the tree.
The phylogenetic tree (Fig. 6) shows that the identified sequences could be divided into three classifications, Proteobacteria, Firmicutes and Acidobacteria.
The identified sequences are published in the EMBL Nucleotide Sequence Database with the accession numbers LT622055-LT622085 outlined in Tables 2 and 3 for bacteria and fungi, respectively.
Selection of representative genes depended on success of the primer design based on the identified sequences.
Based on the identified sequences we constructed for each of the groups a TAR cloning vector.
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