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The identification and segmentation of the neighboring cells were also tested.
Experimental results are presented to demonstrate the superiority of the proposed method on the identification and segmentation of various morphological features with complex boundaries.
HMMgluT performed well in the identification and segmentation of functionally validated glucose transporters.
In the present research work, a hidden Markov model (HMM), that shows a good performance in the identification and segmentation of functionally validated glucose transporters, was constructed.
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The identification and segmentation is based on recovery of the peak wavelength of each spot using knowledge of the relationship between the RGB colorspace and the wavelength of the light.
The correct identification and segmentation of the guide-wire (GW) in a single image can be challenging due to the presence of stent struts and artifacts.
As is the case for many biological applications, the most challenging bottleneck in configuring an image analysis pipeline is the segmentation, defined as the identification and partitioning of the individual plaques in the image.
Furthermore, there was no statistically significant difference in surface area and volume between blastomeres from the two groups (supplementary material Fig. S2), suggesting that the resolution we used for time-lapse imaging was sufficient for accurate identification and segmentation of individual blastomeres.
Automated comparison of mutant 3D data sets with statistically averaged reference data requires manual identification and segmentation of individual organs, regions or tissues (Cleary et al., 2011; Dorr et al., 2008; Kovačević et al., 2005; Wong et al., 2012), a procedure which has only so far proved effective with a subset of embryo organs.
Analysis of kinetoplast and nuclear DNA content of a cell from a micrograph first requires identification of the organelles and segmentation of them from the image, the total signal intensity for each organelle can then be recorded [ 15].
The axial UHR-OCT resolution is sufficient for the imaging, identification and even segmentation of the major corneal layers [ 16, 17].
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