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The standard deviation was calculated from three devices that made on the identical substrate for each condition.
Since our SAS array is formed on a large area, it is possible that each fabrication step contains nonuniformity such as different Pt/Pd film thickness between center and edge of the substrate, which will lead to different sizes and periods of nanodot etch masks on the identical substrate.
The comparison made above illustrates that P450s with drastically different sequences and, presumably, structures can nonetheless employ the identical substrate and catalyze a similar but distinct reaction.
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In a former study by Dalby et al., the opposite was found: namely, randomly patterned poly methyl methacrylate) substrates, though with nanosized, not microsized features, substituted for the role of osteogenic supplements and induced the differentiation of MSCs to osteoblasts, unlike the chemically identical substrates with a perfect translational symmetry.
A common feature of MMP-15 and MMP-24 is that they are membrane-type MMPs sharing an identical substrate, the pro-MMP-2.
As far as comparatively investigated, CYP3A4 and CYP3A5 (84%% amino acid identity!) have almost identical substrate specificities, CYP3A5 with lower rates than CYP3A4.
The A domain binding pocket of HapC is identical to HctE and HctF, and along with the structure of hapalosin, suggests an identical substrate is incorporated (Additional file 3).
We show that all temporal enzyme profiles with the same average enzyme level yield identical substrate degradation– a simple analytical conclusion that can be thought of as an invariance principle, and which we validate experimentally using a β-galactosidase assay.
Mouse and human GrzM exhibit very similar, although not identical, substrate specificities, as is the case for all Grzs.
We found that the human and bovine orthologs have almost identical substrate specificity in their S1 and S2 pockets.
The in vitro acetylation assays verified that zNaa10 is a NAT of the Naa10-type and that it has identical substrate specificity to human Naa10.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com