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Difference in fold expression was observed when the identical specimen was tested by both methodologies as well.
Each specimen can be analyzed at different time points and effects of intervallic treatments can be observed using the identical specimen.
Expression of SEC11L3 mRNA in cancer cells was compared to that of non-neoplastic epithelial cells (ductal, acinar, intestinal and hepatic cells) on the identical specimen and judged using a 0 2 score (0=no staining, 1=weak intensity, 2=intensity comparable to non-neoplastic counterparts).
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Figure 3a, b shows three sets of data for each gene expression (n = 10), expression based of FPKM values in RNA-seq cohort (n = 10), qRT-PCR data from the identical specimens (n = 10) as RNA-seq cohort (relative to actin) and qRT-PCR data of a test cohort (n = 47, relative to actin) of CLL specimens.
This can be attributed in part to the strict adherence to identical specimen collection protocols at both sites.
For example, the monetary value of a small Roman pot can be readily established, but the historical interest of such a pot found in India far outweighs that of an identical specimen found in Italy.
Identical specimen processing protocols were used for all groups.
Identical specimen collection and processing protocols were used for all participants.
Finally, we ensured that positively staining specimens were specifically expressing p53 by comparison to staining of the identical tumour specimens with an antibody that had been preabsorbed with recombinant p53; in all cases, the absorbed antibodies stained at background levels.
For a work of which there are seven other identical specimens, the estimate, £2.2 million to £2.8 million, was steep.
The experiments were performed on 32 quintuplets; each quintuplet was composed of five identical specimens, while the quintuplets differed from each other in the geometry and glass of the specimens.
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