The pKa values were also determined for the hepta(2′-O-methyl)ribonucleotide and the heptamer PNA of identical sequence, as well as for oligodeoxyribonucleotides with different deprotonable bases, viz.
Author's response : The issue of sequence redundancy was dealt with in two ways: First, nearly identical sequences (as well as sequence fragments) were removed; this pruned the input alignment from ~259,000 down to ~65,000 sequences.
Contigs with the identical sequence information as those obtained from PlasmoDB were obtained for five species, however six highly matching hits were retrieved in the P. reichenowi genomic database.
The sequence of each annotated lncRNA was retrieved using Galaxy based on these annotations, and the lncRNA candidates with the identical sequence and genomic locus were regarded as the same transcript.
Rather than paralogs (as they were categorized in the automatic phylome pipeline), we therefore reclassified these identical sequences as being the same gene and obtained a much higher number of 1 1 orthologs.
The automated molecular replacement program BALBES calculated a number of molecular replacement models and output a potential solution utilizing 3RR1 (31% identical sequence) as the search model.
The non-recombined sequences were aligned with ExPASy Decrease Redundancy tool, to identify the identical sequences (referred to as "redundant" sequences).
Briefly, primer (identical sequence as above) was 5' end labeled with 32P, and blocked at the 3' end with ddADP.
Interestingly, strains detected in 2010 had an identical sequence as strain 1026 collected in 2007 tempting us to speculate that strain 1026 might have emerged as a new genetic variant and prevailed through 2010 in the dog population.
