You's team began the experiments by taking an identical population of mouse cells and then starving them of nutrients essentially putting them in a state of hibernation.
23 24 Applying this method, we constructed a summary covariance matrix from the output of two, otherwise identical, population averaged models (using different nesting variables that is, "expedition id" and "individual id").
This pooled population was then expanded, aliquoted, and stored in liquid N2, so that the identical cell population could be used for all subsequent in vivo experiments.
Data calibration requires the measurement of the identical cell population by both techniques.
These records were considered to belong to the same individual, and were matched to the identical municipal population register record.
For calibration, the concentration of the transcription factor STAT5B as well as cell size were determined by confocal microscopy and flow cytometry from the identical cell population, resulting in a linear calibration curve.
For calibration, the identical cell population is measured by a single-cell technique as well as a high-throughput method to determine a subset of matching quantities, defined as the free variables.
Thus, 3D alginate culture seemed to be superior for NC culture as a higher fraction of large NC were revealed after 34 days of culture, compared to the identical cell population cultured in 2D monolayer and with the requirement to passage them three times for the same number of culture days.
Our results demonstrate striking similarities with the innervation of identical populations of hair cells in all the neuromasts forming a stitch in Xenopus, implying that the mechanisms governing the formation of multi-neuromast sensory units is conserved across species [21] [22].
A variation of this general protocol is to start the experimental evolution with identical populations, place them in a similar environment but vary the conditions of adaptation (e.g., [ 20]), the size of the transfer bottleneck or the presence of a mutagen.
