For gene trees, the identical objects are terminal edges, leaves and leaf notations x- y.
DOI: http://dx.doi.org/10.7554/eLife.04803.005 For the results of the total exploratory duration of the identical objects during the acquisition phase for animal experiments of comparison between the young and middle-aged rats, acute stimulation, and chronic studies, see Supplementary file 2A,B,C.
As expected, the four groups of mice explored the two identical objects to the same extent (Figure 4A).
During the acquisition session of the ORT, when the animals are allowed to explore freely the two identical objects presented in the testing arena, WT/E2, and vehicle- and Aβ12-28P-treated APP/E2 mice, equally interacted with both objects as expected of normal mice (data not shown).
Mice from all genotypes spent the same time in exploring the two identical objects to which they were exposed during the sample phase of the test at all ages examined (Supplementary Fig. 1A C, respectively at 3, 6 and 12 months of age).
In the sample phase, the animal was placed in the start arm and left to explore the two identical objects, which were placed on the end of two arms for 5 min.
Novel object recognition memory was operationally defined as the proportion of time animals spent investigating the novel object (or an identical object presented during the training session) and measured in terms of a Preference Index (P.
From this measure, a series of variables were then calculated: the total time spent in exploring the two identical objects in T1, and that spent in exploring the two different objects, F and N in T2.
The total time spent exploring the two identical objects was recorded.
During the sample phase, animals were allowed to explore two identical copies of the sample object for a period of 5 min. The total time spent exploring the two identical objects was recorded.
