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The DA neuronal populations along with general neuronal populations in the human neuron cultures were also greatly reduced by the increased insulin concentration, whereas cell viability of mouse DA neurons (from VM) was unaffected by the higher insulin concentration.
As problems such as pattern recognition, system identification, and system control became difficult to solve using conventional computing methods, the concept of neural networks was inspired by the biological learning and decision-making process of the human neuron system.
Finally, backward propagating (from soma-to-dendrites) signals following steady-state current injection into the soma was also characterized by higher attenuation factor in the human neuron compared with mouse neuron (Fig. 8 E2, E2).
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The mice integrated the human neurons into their complex network of signaling and, despite the alteration, developed virtually identical to the mice that did not receive the injection.
Furthermore we demonstrated that the human neurons expressing TH, also expressed PINK1.
We investigated whether a similar sensitivity to mitochondrial apoptosis was evident in the human neurons as well as the SHSY5Y cells.
After 6 weeks of survival 72% of the GFP+ HNPCs were TUJ1 positive, which may indicate good survival of the human neurons over time.
Pltp is an important modulator of the signal transduction pathways in the human neurons, and is likely involved in neurodegenerative and inflammatory brain diseases [ 21, 22].
This limitation prevents the use of NfH data to estimate the absolute numbers of neurons lost in the human brain, as the human neurons contain ∼24.6 times more NfH than PC12 cells.
The primary human neuron cultures used in this study were at least 90% pure, as measured by MAP-2 and glial fibrillary acidic protein (GFAP) staining, reflecting an environment relatively devoid of astrocytic protection or influence.
This can be implied by our observation that the implanted human neurons express Brn3a at the site where the host expresses Brn3a.
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