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Chilamakuri et al. (2014) compared the target regions of the human kits from three providers (NimbleGen SeqCap EZ v3.0 – 64.1Mb; Agilent SureSelect V4 – 51.1Mb; and Illumina TruSeq and Illumina Nextera original version and protocol – 62.08 Mb).
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To investigate mutations in the human KIT ligand gene (KITLG) gene as a mechanism of 46,XX spontaneous premature ovarian failure.
The human KIT ligand gene, known also as human stem cell factor, is the ligand of the c-kit transmembrane tyrosine kinase receptor (KIT).
The human KIT gene is located on chromosome 4 at map locus 4q12.
Specific primers for PCR amplification of the exons were designed based on the human KIT genomic sequence (Genebank accession number AC006553, Human chromosome 4) (Table 1).
[ 10- 12] These observations in mice suggest that mutations in the human KIT gene (MIM 164920) might be a cause of premature ovarian failure in women.
However, this gene is not part of the tyrp1 paralogon, as kita is found on LG 20 and not on LG 7 in zebrafish and the human KIT is found on chromosome 4 and not on chromosome 9.
The lower limits of detection for the ELISA human kits were 15.6 pg/mL for IL-6 (BD Biosciences), 3.9 pg/mL for IL-17A (eBiosciences), 15.6 pg/mL for IL-22 (eBiosciences), and 15.62 for IL-23p19 (eBiosciences) levels were measured at 48 h and 24 h after restimulation by human kits.
The kits for nonhuman species use similar bait designs and protocols to the providers' human kits.
The discussion of the characteristics of each platform will focus on the solution-based human kits; the performance of kits for other species has not been subjected to the same level of comparison.
However, the ELISA human kit used did not detect plasma TGF-β1 concentration.
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