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Green dotted line represents the healthy threshold of 1.32 CTCs/mL above which patients are defined as positive for CTCs.
Using our platform integrated with these workflows, we analyzed 32 non-small cell lung cancer (NSCLC) and 22 breast cancer patient samples, yielding 60 to 100% of the cancer patients with a cell count over the healthy threshold, depending on the detection method used: respectively 77.8% for automated, 60-100% for cytology, and 80% for immunostaining based enumeration.
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A healthy threshold can be defined from the healthy donors as 1.32 CTCs/mL (average + 2 SD).
Nevertheless, the trend of the treated group approaching the contralateral healthy nerves threshold is a good evidence of reinnervation.
We believe, therefore, that the mouse tolerates a higher withdrawal threshold on the healthy side in order to prevent loading of the inflamed side.
We found that the dynamics of the antigen-specific T-cell subpopulation after anti-CD20 therapy was reproduced by reducing the Keff threshold below the healthy standard (<1,000 cells; e.g. 850 cells), independently of the αreg parameter (αreg values were in the low range of autoimmune regime; Figure 6).
The highest value of the healthy control group was set as threshold value for positivity (QDA value > threshold: 'QDA-positive') and negativity (for QDA value ⩽ threshold: 'QDA-negative') of other samples.
Further empirical parameter adjustments were made to improve the fit of recovery of excitability, SDtc, TE, and current-threshold-relationship in the healthy controls [14].
The threshold value was determined from the mean value plus twofold SD from the healthy group.
Furthermore, the distribution of T2 signal within the healthy population is skewed and hence high T2 values, even below 92 ms (the clinical threshold), are rare in healthy individuals [Bartlett et al., 2002].
This result also suggests that RF is less dependent on the choice of the threshold for classifying healthy and affected animals, providing larger stability to the classification.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com