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Fifty one of these SLESs in the minimal and two in the YP medium were fixed by using the global modifications found for GNGs.
We followed this analysis by allowing for up to two simultaneous gene/reaction suppressions for each GSL and considered only the global modifications.
All the global modifications for which we did not find any supporting evidence using the methods mentioned above, were not incorporated into the model and were just added to the list of modifications with no corroborating evidence.
Overall, upon including only the global modifications for which a supporting evidence was found, we could fix three auxotrophy inconsistencies for essential genes as well as one for SLs under both minimal and YP media (see Additional file 2 for details).
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The number of 33 design variables consists of nine defining the global modification function and 24 that altogether define four local modification functions.
Yet, the relations between transverse cracking and the global modification of resistivity is still unclear that makes difficult to interpret these non-destructive-testing results.
FabLEM has been useful to track both the localization of modifications and the global modification level in the nucleus.
Hence, these global modifications induced by zebularine open and relax the structure of chromatin, increasing the accessibility of promoters to transcriptional machinery.
Based upon these global modifications, a highly potent peptide ligand 12 was discovered with an IC50=1.68 nM, evaluated by ELISA binding essay.
Based upon these global modifications, a highly potent peptide ligand 9 was discovered with a Kd = 17 nM, evaluated by Biacore binding assay.
To introduce the elastic behavior of fractures into the model, initial global modifications involved elevating the aquifer permeability, elevating fracture permeability in x direction by a factor of 10 and reducing fracture permeability in z direction by a factor of 10 to compensate for the elasticity of microfracturing observed in the cores.
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