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The number of 33 design variables consists of nine defining the global modification function and 24 that altogether define four local modification functions.
Yet, the relations between transverse cracking and the global modification of resistivity is still unclear that makes difficult to interpret these non-destructive-testing results.
FabLEM has been useful to track both the localization of modifications and the global modification level in the nucleus.
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Fifty one of these SLESs in the minimal and two in the YP medium were fixed by using the global modifications found for GNGs.
We followed this analysis by allowing for up to two simultaneous gene/reaction suppressions for each GSL and considered only the global modifications.
All the global modifications for which we did not find any supporting evidence using the methods mentioned above, were not incorporated into the model and were just added to the list of modifications with no corroborating evidence.
Overall, upon including only the global modifications for which a supporting evidence was found, we could fix three auxotrophy inconsistencies for essential genes as well as one for SLs under both minimal and YP media (see Additional file 2 for details).
An important factor for the stability of global modification patterns in the model is the recruitment efficiency.
Additionally, it allows the extraction of global modification profiles across and within each of the input samples thus allowing the inference of correlations between certain modification patterns and any conditions indicated by the input samples.
The changes in global modification levels can be monitored by FRAP or by comparing the nuclear/cytoplasmic intensity ratio measurements.
Hence, these global modifications induced by zebularine open and relax the structure of chromatin, increasing the accessibility of promoters to transcriptional machinery.
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