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The generated plasmids was packaged into HDAdV following previous report (Yang et al., 2017).
The generated plasmids were sequenced.
The generated plasmids were transformed into the hmp deficient strain AG1000.
The generated plasmids were designated M1, M2, M3, M1+2, M1+3, M2+3 and M1+2+3 respectively.
In the generated plasmids (checked by DNA sequencing), the transcriptional fusion is included in a cassette labeled by the spc marker and delimited by the front and back regions of the B. subtilis lacA locus.
All the generated plasmids were DNA sequence-verified.
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The overexpression of the anti-HER2/neu mini-antibody-barnase fusion protein decreased the intensity of fluorescence of HEK 293T cells co-transfected with the generated plasmid and a plasmid containing the gene of enhanced green fluorescent protein (pEGFP-N1), in comparison with the intensity of fluorescence of HEK 293T cells transfected with pEGFP-N1, in the absence of tetracycline in the medium.
The correct sequence of the generated plasmid was confirmed by nucleotide sequence analysis.
The generated plasmid pGFP103464 was transfected into P. berghei and a parasite strain, PbGFP103464, was established.
We demonstrated that this standard has the same amplification efficiency of the generated plasmid standard containing the target LSU gene.
The generated plasmid DNA was used for transformation of Mach-1 cells (Invitrogen) and isolated from selected clones.
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