After the initial reconstruction was generated, gaps in metabolic pathways necessary to produce biomass components and key virulence factors were filled by cautious literature mining, BIOLOG substrates utilization assays, and BLAST searches on homology and protein sequence similarity analyses [ 89, 90].
We used a long-forked substrate as a control for WRN helicase activity as described previously and modified the substrate generating gaps of different sizes on the translocating strand.
Once washed with 700 μL PBS/0.1% NaN3, pellets were treated with 100 μL Intra Prep™ permeabilization reagent (Beckman-Coulter, Krefeld, Germany) for 10 minutes at room temperature in the dark to generate gaps in the membranes.
Ductal breast cancer accesses the lymphatics in bulks generating gaps in the lymphendothelial cell (LEC) wall that serve as entry gates for the tumour.
Press-fit fixation can potentially generate gaps between the cup and the acetabular bone.
In motion distorted data, some areas of tissue might be sampled multiple times, while others might not be sampled at all, generating gaps in the data.
This supports a model where MMR generates gaps in the DNA (Mojas et al, 2007) that block replication, which leads to DSBs at collapsed replication forks in the second cell cycle after treatment (Ochs and Kaina, 2000; for review see Kaina et al, 2007).
The smaller size of the CATIE Type 2 population reduces the number of observed recombinations, causing markers to co-locate and generating gaps between clumps of markers.
In particular, as a lobe always emerges opposite a neck, how can each cell control its growth tightly enough not to generate gaps between growing cells?
A substrate-induced potential thus generated can break the symmetry of the honeycomb lattice and generate gap in electron system.
