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The gels were normalized using the ladder and common bands.
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The grouped gels were normalized using standard settings of the programme and compared.
Protein spots were matched and gels were normalized using the internal standard present in all gels.
Gels were normalized using an internal ROX-labeled size standard included in each lane.
Briefly, protein spots were automatically detected first and then refined manually; subsequently the spots in the gels were normalized by using of relative volume (%Vol), which is the ratio between its volume and the sum of all the spots volume in the gel were calculated and used for quantitative comparison and then matched.
The resulting lysates were normalized using the Bradford assay (Bio-Rad) and 20 µg of total protein was resolved on 12% Tris-Tricine gels and analyzed by immunoblotting.
The values were normalized using the LOWESS algorithm.
The filtered data were normalized using Lowess Smoother.
The microarray data were normalized using a median centering procedure.
The fluorescence intensity values were normalized using LOWESS normalization.
Expressed data were normalized using the Median normalization.
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