The presence of these fusions indicates the acute promyelocytic leukemia (APL) subtype of AML, which is particularly sensitive to treatment with all-trans retinoic acid [ 2].
These cells displayed telomere elongation comparable to strains expressing WT Ccq1 and the Trt1-Tpz1 fusindicatingathat that fusion of Trt1 with Tpz1 bypasses the need for Ccq1 in telomerase recruitment.
These data were confirmed by qRT-PCR analysis of independent lines carrying the 4x:: GUS fusion, indicating that the multimerisation of the DOF1 site per se is not sufficient to drive gene expression in guard cell.
Finally, patch clamp assays of HEK293 cells heterologously expressing the AtCNGC10-GFP fusion indicated that the AtCNGC10 channel transported K+.
We observed a fraction of GFP-taperin in the mouse nucleoplasm following fusion, indicating that it migrated from the human nuclei in the heterokaryon (Fig. 3A).
Both plots indicate that the fusion point is randomly distributed along sequence length.
If the detection result of S U i (i = 1, 2, …, K T 1 i > λ1 + Δ, sends the local decision D 1 i = 1 to the fusion center indicating that PUs exist; if T 1 i < λ1 − Δ, sends the local decision D 1 i = 0 to the fusion center indicating that no PU exists; if λ1−Δ≤T 1 i ≤ λ1 + Δ, nothing will be sent.
The TRITC-dextran content of the phagosome was diluted by the fusion event, indicating that the source of the fluid influx was at least partly unlabeled (Figure 10 and Movie S12).
A protein with loss of the terminal region alone was not as highly activated as the fusion proteins, indicating that the fusion partners are essential.
Excess amounts of anti-HER2 affinhibitedibindinginding of the fusion protein, indicating that binding of the fusion protein is mediated by the specific interactions with HER2 receptors.
Studies comparing either patients with and without the TEL-AML1 fusion or TEL-AML1-positive cell lines with small hairpin RNA-mediated knock down of the fusion protein indicated expression differences for genes involved in differentiation, apoptosis and immune responses.
