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In fact, more than 10 of the 30 genes required for the functioning of the terminal portion of the mitochondrial respiratory chain have been found to be mutated in several devastating human diseases such as Leigh syndrome, an early onset encephalomyopathy, myo-, neuro-, gastrointestinal encephalopathy (MNGIE syndrome) or Kearns Sayre syndrome (KSS).
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Our results also support the function of the terminal FM-sweep for accurate ranging.
To characterize the function of the N-terminal H1 helix, we constructed an N-terminal deletion mutant, Png1p-ΔH1 (33–363 aa).
A possible explanation is that the N-terminal extension has been shown to assist the folding of βB1 (Leng et al., 2014) and thereby the N-terminal tagged GFP might interfere with the function of the N-terminal extension.
This rather low number may be related to the fact that the proteins were C-terminally tagged, which masks the function of the C-terminal PTS1 sequences.
The N-terminal domain contains the bifunctional active site, but the function of the C-terminal domain is unknown.
Subtle light-induced structural destabilization was observed for the region between residues 28 and 44 of the N-terminal domain, which suggests that illumination also affects the function of the N-terminal domain.
Second, the function of the N-terminal β-barrel domain is independently involved, because 15-LO1 with an N-terminal β-barrel domain truncation is still able to restore HIF-1 α in the presence of an intact C-terminal catalytic domain.
The functions of the C-terminal proline-rich linker (L), the Dsg-specific repeated unit domains (RUDs) and the desmoglein terminal domain (DTD), which are all only present in desmogleins, are not clear (Dusek et al. 2007b).
Although there is an abundance of information regarding the function of the C-terminal pocket domain of Rb, there is little known about the function of the amino-terminal domain of Rb family members [40].
The N-terminal domain is responsible for binding to HSP90 [ 82] whereas the function of the C-terminal domain remains unknown.
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