The mutants were co-expressed with TipE subunit ensuring the functional expression and currents were recorded in the same condition as that of wild type DmNav1.
We further examined the functional expression of Ct-OATP1B3 using HCT116 cells transiently expressing each OATP1B3 isoform.
Patch-clamp analyses performed on GFP-expressing interneurons navigating in slice culture supported the functional expression of GlyR α2 homomers.
Thus, these results show apparent inconsistencies regarding the functional expression of Ct-OATP1B3.
Thus, the functional expression analysis of Ct-OATP1B3 was performed using human LS180 and HCT116 cells (colon cancer).
Certainly, the functional expression of rat vascular α1-adrenoceptor subtypes depends on several factors, including age [25].
E. coli (TLL) was genetically engineered and grew easily to a high cell density with the functional expression of TLL.
In vitro enzyme assay confirmed the functional expression of XRMUT, XDH and XK in recombinant S. cerevisiae strains.
When the functional expression of Rubisco in E. coli reached a "saturated" level, several point mutations could hardly improve its expression level further.
To maximize the functional expression of Rubisco, two strong promoters (T7 and trc) and three E. coli hosts (BL21 DE3), DH5α, and HB101) were chosen.
To determine the functional expression of the cloned PHA biosynthetic genes in vivo, PHA biosynthesis was carried out with transformant C. necator.
