Calvin is able to change the function of the boxes by rewriting the label and flipping the box onto another side.
The function of the SOCS box is the recruitment of the ubiquitin-transferase system.
This mutant also retained its early-firing profile in VPS13 locus, indicating that the Fkh1/2-binding site can substitute for the function of the B3 box (Fig 4I).
In their appeal, they have provided a greater response to that but are still missing/avoiding the point and lacking in the validation experiments that could support the importance of the function of the RGG box.
In this study, we provide the first clues as to the function of the F-box protein, FBXO28.
This indicated that the C-terminal domains determine specific protein-protein interactions in important biological processes and critically define the function of the F-box gene.
To paraphrase my previous summary of that issue, in order to support the importance of this work investigating the function of the Aven RGG box, it should be demonstrated that the RGG box is required for the anti-apoptotic (or other) biological functions of Aven in affecting cancer progression.
While it is impossible to validate the function of the putative TATA boxes in silico, it should be pointed out that the TATA box found around 400 nucleotides downstream of the 5′ LTR end is highly conserved in all the 14 complete Atlantys LTRs isolated from the other Oryza species (Fig. 5).
To investigate the function of the putative Dof-boxes in the AtACBP6 5′-flanking region, EMSAs were performed using double-stranded biotin-labelled DNA Probes 1, 2 and 3 representing each of the three Dof-boxes (−490/−486, −421/−417 and −353/−349, respectively).
One study showed that one regulator of R-genes, SGT1 (suppressor of G2 allele of SKP1), is essential for the function of the Skp1-cullin-F-box protein (SCF) E3 ubiquitin ligase complex that targets proteins for degradation by the 26S proteosome.
