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This file contains the full scans of the immunoblots used in the figures.
Maximal filling times were 250 ms for the full scans and 60 ms for the MS/MS scans.
Data are representative of two independent experiments with similar results; images were cropped and the full scans are shown in Supplementary Fig. 2b.
The experiment in k was repeated two times with similar results; images were cropped and the full scans are shown in Supplementary Fig. 2d.
Partial and rate-limited scans are simulated by subsampling some proportion of the full scans and distributing their event times uniformly over the duration of the flow being analyzed.
The full scans were averaged from five microscans, and the MS/MS scans were averaged from two microscans.
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A global-optimal model and some local-optimal models were selected by OMWPLS screening through the full scanning range.
The mass spectrometer was programmed to acquire, by data-dependent acquisition, MS/MS from the top 20 ions in the full scan from 400 to 1200 m/z.
Moreover, the rotational errors between axes are measured to ensure the accuracy of the calibrated AFM within the full scanning range.
The MS was operated in the full scan mode scanning the mass range of 30 700 amu.
Mass spectra were recorded using the full scan mode in the range of 200 800 Daltons.
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