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For the introns (MGF, PRKC, SPTBN and THY), the number of base pairs for the full alignments and those remaining after removal of the poorly aligned positions with Gblocks, together with the number of positions in intronic and exonic regions, are indicated for each gene in Table 1 (also see Additional files 1 and 2 for intron alignment before and after Gblocks).
This conservation is shown in Figure 7. (The full alignments are shown in Data S4).
The full alignments for the PDZ domain family (PF00595) and chorismate synthase family (PF01264) were downloaded from the Pfam database (http://pfam.sanger.ac.uk/, Pfam 21.0) [21.0
Analyses were conducted on the full alignments as the exclusion or weighting down of large indels or repeat regions was found not to significantly effect the overall topology (see also [5]).
The full alignments may be obtained from TreeBase.
We also ran INTREPID on these reduced alignments as well as the full alignments for these 71 families.
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RNI was first aligned to Homo_7 using these methods, and adjusted manually, and then aligned to the full alignment of all human PRAMEs guided by the Homo_7 alignment.
Number of positions: in the full alignment (column 1), after elimination of poorly aligned positions by Gblocks (column 2), in the remaining exonic parts (column 3) and in the intronic regions (column 4).
The remaining polyserine segment was separately aligned using t-coffee and re-inserted into the full alignment.
The Bryopsis and Tydemania cpDNAs were aligned using the progressive Mauve algorithm in Geneious using the full alignment option and automated calculation of locally collinear block (LCB) scores [ 58, 79].
The complete mt genomes in each cluster were aligned with Mauve v.2.3.1 (Darling et al. 2010) using the full alignment option.
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CEO of Professional Science Editing for Scientists @ prosciediting.com