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Finally, the relative abundance for each population was determined as the fraction represented by each population of the total summed coverage values.
Homogenates were centrifuged at 160,000 g for 20 min, and the supernatant fraction, representing the cytosolic fraction, was removed and stored at −80°C.
The main peak fraction, representing the purified promutoxin, was pooled, lyophilized, and stored at –20°C.
The resulting supernatant fraction representing cytosol and light membranes was collected.
The sub-G1 fraction (representing apoptotic cells) was also determined after transduction with virus and staining with PI (Sigma Chemical Co).
The surviving fraction represents the ratio of the viable TiO2-treated cells relative to that of the control groups.
The supernatant fraction represents the cytoplasmatic fraction.
The 60% fraction represented the epithelial cell enriched population.
The HS/T fraction represents the transmembrane cytoskeletal fraction of normal dysferlin and decreases with advanced stages of dementia.
A fraction representing 10% of the total sample volume was removed and placed on ice for further purification (see below).
The coarse fraction represents exposures from the nasal cavity to tracheobronchial tract, and ball-rolling has the highest exposure level.
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