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A Superscript III Reverse Transcriptase kit was used to generate the cDNA from the same RNA extracted for the RNA-seq analysis, and qRT-PCR was conducted using an Agilent MX3000P (Agilent, USA) with the following programmed conditions: 94 °C for 2 min; 40 cycles of 94 °C for 20 s, 57 °C for 20 s, and 72 °C for 30 s.
The chromatographic separation of nine sweeteners and internal standard was performed using the following programmed gradient profile of 0 4 min, isocratic at 100% B, 4 14 min; linear gradient from 100% B to 53%B; 14 20 min, linear gradient from 53% B to 0% B; 20 24 min, isocratic at 0% B, 24 26 min, back to 100% B, 26 36 min 100% B (column equilibrating).
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All reactions were carried out with the following program: 30 s at 95°C, followed by 44 cycles of 95°C for 3 s and 60°C for 34 s.
The following program was used: 98 °C for 3 min, followed by 40 cycles of 98 °C for 15 s, 58 °C for 30 s, and 72 °C for 30 s.
After initial denaturation for 4' at 94°C the following program was run for 35 cycles: 30″ 94°C, 3' 60°C, 1' 72°C followed by an extended elongation for 7' at 72°C.
"THE following program was filmed on location at a public high school.
News shows should probably come with a spoiler alert: the following program contains images and language that seem to jump to a foregone conclusion.
To overcome the fear of mesh collaboration, I recommend the following program.
In the video, we marked the following program error and participant mistakes with red labels.
Now, we consider the following programming: min_{x}sum_{j=1}^{n}|b_{j}-a_{j}x|.
Hence, determining the optimal prices is done by solving the following programming problem: (11a).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com