10 μM of each primer was used for real-time PCR with the following program: 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s and 55 °C for 30 s.
Following a 10 minute denaturing step at 95°C, the following program was used: 95°C for 30 s, 55°C for 45 s, and 72°C for 60 s, repeated for 24 cycles.
After initial denaturation for 4' at 94°C the following program was run for 35 cycles: 30″ 94°C, 3' 60°C, 1' 72°C followed by an extended elongation for 7' at 72°C.
The following program was used for denaturation and amplification of cDNA: 3 min at 95°C, followed by 40 cycles of 15 sec at 95°C and 45 sec at 60°C.
"THE following program was filmed on location at a public high school.
The following program developed by Terri MacKenzie, SHCJ can help materialize such groups.
News shows should probably come with a spoiler alert: the following program contains images and language that seem to jump to a foregone conclusion.
For example, the following program copies all the input files to the output, except for records that say '@include filename'.
RequirementsIn order to graduate with a minor in Creative Writing, students must complete the following program of six 5-unit courses for a total of 30 units.
For example, the following program reads the current date and time into the variable current_time, using the date utility, and then prints it.
Accordingly, EPA has established the following program goal, expectations, and program management principles to assist in the identification and implementation of appropriate remedial actions.
